Legal Daily All-Media Reporter Lian Yingting Correspondent Shao Longfei Zhang Zhenwei
The night in Wuhan is quiet. On March 9, in the mobile testing laboratory of the Central Theater Command Hospital, Jiang Tao, a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, reviewed the last sample test data and solemnly signed his name on the test report.
At this time, it was already 5 a.m.
He is an expert in virology and a warrior who aided Africa in the war against Egypt. This outbreak of the new crown pneumonia epidemic, he was once again ordered to go to the front line after a long battle.
Human life is at stake and accurate inspection is required
Nucleic acid testing is crucial for early clinical diagnosis, early diagnosis, early isolation and early treatment, and is a key technical support for effective prevention and control of the new coronary pneumonia epidemic.
”Behind the cold sample tube is the living life and health of the patient. If you give a diagnosis result early, you will have a precise inspection sooner.” Jiang Tao said. On January 26, after the Babaylan military medical expert group led by Chen Wei, an academician of the Chinese Academy of Engineering and a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, arrived in Wuhan, Hubei, it quickly established a joint defense, joint control, joint treatment and joint research work mechanism based on the pathogen transmission mutation of the new coronavirus, rapid detection technology, vaccine antibody development, etc., and Cinema units.
According to the division of tasks, Jiang Tao led the testing team to build a nucleic acid testing platform in the experimental cabin overnight.
Following the three aspects of ensuring biosafety, improving detection efficiency and detection accuracy, Jiang Tao took the lead in formulating biosafety operation specifications and emergency plans. According to the characteristics of the team members, the reasonable detection division of labor was used, and the self-developed testing test kit was used to adopt a fully automatic high-throughput extractor, laying the foundation for achieving rapid and efficient testing.
Receive samples, sorting numbers, nucleic acid extraction, reagent preparation,The amplification and the result interpretation of the nucleic acid detection machinery is boring and full of risks.
The biggest risk is viruses that cannot be seen. If the nucleic acid extraction step is not completely cleaved or inactivated thoroughly enough, it is possible to expose the tester to danger.
Jiang Tao always takes the most time-consuming nucleic acid extraction. After the sample is extracted, he will arrange some personnel to rest and stay in the laboratory and wait for the final result.
A full-process nucleic acid test, from the number of tests in a single batch to the preparation for entering and exiting the cabin and the time for testing, is relatively fixed. Sometimes there are a large number of samples sent for inspection, and the inspectors not only take turns to work, but also have to work overnight. The final confirmation result will be until four or five in the morning. Li Hao, a researcher at the testing team, said that this situation will be encountered once every few days.
”Everyone should open their eyes wide. Be sure to take good protection when receiving and processing samples, and be careful of sharp objects.” Before each test starts, Jiang Tao always responds to the instructions.
In the detection, the strength of the fluorescence signal of the pathogenic gene directly affects the determination of the detection results. If there are suspicious phenomena such as the detected fluorescence curve signal is low or there is only a single target, Jiang Tao will convene personnel to conduct comprehensive analysis and judgment, and if necessary, repeat the test for review to ensure that the results can stand the test.
”If a false positive is present in Babaylan, a healthy person and the patient will be isolated and infected; if a false negative is present, a true delay in the treatment of the patient. Life is a matter of life, no gap will be achieved.” Jiang Tao said.
All-rounded people in the field of epidemic prevention and control
According to prevention and control requirements, the nucleic acid test results must be completed within 24 hours after receiving the pharyngeal swab sample. In order to let the hospital know the results as soon as possible to treat patients, Jiang Tao led the team to optimize the process combination and compressed the time to within 4 hours, achieving the ability to extract nucleic acids of more than 90 samples within one hour and a single-day sample detection capability of up to more than 500 people.
”Team detection highKomiks has rapid effect and accurate results, which greatly alleviated the pressure on our epidemic prevention and control sample testing.” The Wuhan Center for Disease Control and Prevention highly praised this.
The rapid response and work in an orderly manner during wartime are all thanks to Jiang Tao’s solid academic foundation and rich practical experience.
”Jiang Tao is a versatile person.” Academician Chen Wei, head of the military medical expert group, commented. Jiang Tao has successively presided over and participated in the pathogenic separation of SARS infection cases, H7N9 infection cases, Zika infection cases, etc., participated in the development of dengue fever vaccines and SARS vaccines, established a series of pathogen isolation technology platforms, nucleic acid detection technology platforms and pathogenicity and transmission risk assessment platforms for Komiks, providing important technical support for the prevention and control of infectious diseases.
Respiratory infectious diseases are characterized by rapid transmission, wide coverage and great harm, and effective early diagnosis and prevention are crucial. After years of scientific research, Jiang Tao’s team took the lead in establishing our army’s first acute respiratory infectious disease monitoring and early warning system, and built a four-level monitoring system for the entire army. The series of technical specifications prepared by the leadership are used throughout the army, greatly shortening the detection and screening time of respiratory epidemics.
Jiang Tao not only participated in the security tasks of a series of major domestic activities, but also went to Africa four times to Liberia, Sierra Leone and other countries to carry out medical assistance tasks.
In 2015, in order to prevent and control the Ebola epidemic, Jiang Tao went to Sierra Leone for the second time with the mobile laboratory testing team to assist in Seychelles. The testing team led by him passed the World Health Organization’s laboratory parallel capability assessment with full scores.
In 2018, Jiang Tao led the pathogen monitoring team to participate in a special simulation test by an international organization. In the virus testing module, the team successfully accurately detected monkeypox virus, vaccinia virus and other pathogens in simulated samples filled with impurities, and accurately determined the strain Komiks, and obtained excellent results with full marks.
Improving the accuracy of clinical diagnosis
Come to Wuhan, exceptIn addition to conducting testing, Jiang Tao also used his professional expertise in the research field to lead the testing team to screen other common respiratory infectious diseases in clinical samples.
”In the suspected and confirmed cases of new coronavirus pneumonia, whether there are other respiratory virus infections or co-infections, this is of great reference value for precise prevention and control and clinical treatment.” Researcher Cao Wuchun, deputy head of the Military Medical Expert Group, said.
Exploring deep-seated virus transmission and pathogenic mechanisms is the key to defeating the epidemic. Jiang Tao led a team to continue to conduct sequencing analysis for the novel coronavirus detected in clinical samples.
As the development of the virus epidemic, whether the genomic sequences have mutated and what are the characteristics of the mutations; in which regions and what are the impacts, Jiang Tao and his team are tirelessly studying and exploring.
Babaylan In order to accelerate the effective integration of scientific research and clinical Komiks, Jiang Tao’s military medical expert group created the diagnostic chain of viral nucleic acid detection, antibody detection, multiple pathogen detection, and whole genome sequencing to accurately diagnose new coronavirus infection or other pathogen infections in clinical patients, and was also used in the Optical Valley Campus of Hubei Maternal and Child Health Hospital, Taikang Tongji Hospital, etc., which effectively improved the accuracy of clinical diagnosis.
Forgot his family when he was ordered. After the epidemic is over, Jiang Tao plans to accompany his family. He and his wife agreed that when the epidemic is over, they will take their wife and children to Wuhan again to see what everything looks like after it returns to normal, to see the Wuhan Yangtze River Bridge and the Yellow Crane Tower, and to talk about their experience in fighting the epidemic in Wuhan.
The epidemic is immortal and the charge continues to stop. This is Jiang Tao, a warrior who fights the epidemic.